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Analysis of the <t>BAG1/BAG3</t> triage system. a Western blot analysis of the expression of BAG1 in the RIPA-soluble protein fraction after 24 h ( n = 3) and b 72 h ( n = 4). c Analysis of the expression of soluble BAG3 after 24 h ( n = 4) and d 72 h ( n = 4). e. Analysis of BAG3 in the insoluble fraction after 72 h of treatment ( n = 3). Data were normalized against Vinculin as loading control. For the insoluble fraction, the same amount of proteins quantified in the respective soluble fractions were considered. Data are expressed as fold increase ± S.E.M and compared to normalized control, shown as dashed line. Statistical significance was determined after one-way ANOVA with Dunnet’s multiple comparison test of each treatment against control. *** p < 0.001; ** p < 0.01; * p < 0.05
Bag1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis of the BAG1/BAG3 triage system. a Western blot analysis of the expression of BAG1 in the RIPA-soluble protein fraction after 24 h ( n = 3) and b 72 h ( n = 4). c Analysis of the expression of soluble BAG3 after 24 h ( n = 4) and d 72 h ( n = 4). e. Analysis of BAG3 in the insoluble fraction after 72 h of treatment ( n = 3). Data were normalized against Vinculin as loading control. For the insoluble fraction, the same amount of proteins quantified in the respective soluble fractions were considered. Data are expressed as fold increase ± S.E.M and compared to normalized control, shown as dashed line. Statistical significance was determined after one-way ANOVA with Dunnet’s multiple comparison test of each treatment against control. *** p < 0.001; ** p < 0.01; * p < 0.05

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Proteostasis network response to environmental chronic stress: linking survival to protein aggregation in a human neuroblastoma cellular model

doi: 10.1007/s00018-025-05884-6

Figure Lengend Snippet: Analysis of the BAG1/BAG3 triage system. a Western blot analysis of the expression of BAG1 in the RIPA-soluble protein fraction after 24 h ( n = 3) and b 72 h ( n = 4). c Analysis of the expression of soluble BAG3 after 24 h ( n = 4) and d 72 h ( n = 4). e. Analysis of BAG3 in the insoluble fraction after 72 h of treatment ( n = 3). Data were normalized against Vinculin as loading control. For the insoluble fraction, the same amount of proteins quantified in the respective soluble fractions were considered. Data are expressed as fold increase ± S.E.M and compared to normalized control, shown as dashed line. Statistical significance was determined after one-way ANOVA with Dunnet’s multiple comparison test of each treatment against control. *** p < 0.001; ** p < 0.01; * p < 0.05

Article Snippet: Primary antibodies utilized were: PARP-1, Cell Signaling Technology, 9542; BiP/Grp78, Abcam, ab32618; p-eIF2⍺, Cell Signaling Technology, 9721; eIF2⍺, Cell Signaling Technology, 9722; Ubiquitin, antibodies.com, A85455; BAG3, Invitrogen, MA5-32706; BAG1, Proteintech, 19064–1-AP; LC3, RBC Lifescience, PD014; p62/SQSTM1, Cell Signaling Technology, 8025; pTDP-43, Proteintech, 80007–1-RR −1-AP; TDP-43, Proteintech, 12892–1-AP; β-Actin, Sigma-Aldrich, A5316; Vinculin, Sigma-Aldrich, V9131.

Techniques: Western Blot, Expressing, Control, Comparison